Scientific summaries

Clinical evaluation and user experience of Vitrolife Labware dishes

Document ref and version: Five dishes specifically developed and toxicity tested for IVF were evaluated by two clinics regarding clinical performance. The results at both clinics show that Vitrolife Labware are safe and support treatment success when used for clinical IVF. In addition, twenty clinics gave the dishes a higher score of usability than their traditional dishes showing that the new dish design is at least as user friendly as other dish types commonly used for IVF.

Comparison of cover oils - OVOIL gives both more good and top quality embryos

Document ref and version: In a study by Sifer et el, microdroplet embryo cultures covered with OVOIL from Vitrolife gave a significantly increased mean number of good and top quality embryos at day 3, compared to other commercial available oils. This difference may be explained by the origin of raw materials and the difference in quality tests performed by each company.

Embryo culture in G-Series results in significantly higher cell numbers and implantation rates compared to culture in HTF

Document ref and version: A study by Kleijkers show that besides cell numbers and implantations rates, gene expression and certain pathways in preimplantation embryos are also affected by culture medium.

EmbryoGlue improves pregnancy and implantation rates. Results from a meta-analysis on almost 10,000 embryo transfers

Document ref and version: A meta-analysis of Japanese studies, including almost 10,000 cycles shows that EmbryoGlue improves implantation resulting in increased pregnancy rates confirming findings from the Cochrane reports.

G-Series culture media improve blastocyst development

Document ref and version: Data from two studies presented at Annual Meeting of the Japanese Society of Clinical Embryologist show that good quality embryo rate and blastocyst development can be improved when using G-Series culture media (G-IVF, G-1 and G-2) from Vitrolife compared to a HTF-based non-hyaluronan containing media.

G-Series culture media shown to increase blastocyst development compared to control

Document ref and version: In a study by Matsuoka et al, embryo culture was done either Vitrolife’s G-Series™ culture media or a mix of sequential culture media (control). The results show that good-quality blastocyst development was more frequent after culture in G-Series media containing hyaluronan.

G-TL supports blastocyst development in large volume

Document ref and version: In a study presented at the ACE meeting in the UK, Shaw and co-workers compared single step time-lapse medium (Vitrolife, G-TL) with sequential series media (Sage, Quinn’s advantage) in large volume culture as well as micro drop culture. They found significantly higher day 5 blastocyst development rate in the G-TL group in both large and small volume culture.

iDAScore Science publications and selected abstracts

Document ref and version: REF19196

New formulations of slowfreezing solutions for cleavage stage embryos result in outcomes similar to after vitrification

Document ref and version: A study by Fang et al, found that FreezeKit Cleave and ThawKit Cleave significantly improved survival rates, clinical pregnancy rates and implantation rates. In fact, a survival rate of above 90% using the new formulations of solutions is very similar to that obtained after vitrification.

Optimised clinical outcomes with blastocyst culture and cryopreservation using Vitrolife G-Series culture media

Document ref and version: A study by Zhu et al demonstrate that extended culture before cryopreservation allows for selection of viable embryos with a high chance of implantation after warming. The high blastocyst formation rate (73.8%) using Vitrolife culture media, together with an efficient cryopreservation program resulting in survival rates of >96% should eliminate any earlier concerns about blastocyst culture and cryopreservation.

Rapid-i is an effective system for human embryo vitrification at both the blastocyst and cleavage stage

Document ref and version: In this study, Desai et al, conclude that “Rapid-i offers an excellent alternative to existing open vitrification devices for both day 3 embryos and for blastocysts” at the same time as it prevents direct contacts between embryos and liquid nitrogen.

STAR trial: VeriSeq™ PGS

Document ref and version: The STAR trial: A randomised controlled trial (RCT) comparing pregnancy rates following VeriSeq™ PGS versus standard morphology for elective single embryo transfer (eSET)

Sperm preparation with SpermGrad show positive effect on average telomere length, sperm motility and DNA fragmentation

Document ref and version: Yang Q, et al, found significant correlations between sperm telomere length and the quality of early embryonic development. Density gradient centrifugation using SpermGrad (Vitrolife) enrich for sperm with high mobility and low DNA fragmentation and is the preferred technique for selection of sperm with longer telomeres.

SpermFreeze Solution™ studies show equal performance compared to egg yolk containing medium

Document ref and version: A study by Tekcan M, et al, shows that SpermFreeze Solution™ without egg yolk performs just as well as a yolk containing medium and is free from undefined substances.

Time-lapse systems by Vitrolife - Selected studies about embryo development and outcome

Document ref and version: Provided is a list of some of the studies about embryo development and clinical outcomes using EmbryoScope and EmbryoScope+ time-lapse systems.