RapidVit™ & RapidWarm™ Oocyte
Specialised media for oocyte vitrification.
Description MOPS buffered media containing cryoprotectants and human serum albumin.
Purpose For vitrification and warming of oocytes.
Application For use in sequence after equilibration at +37°C and ambient atmosphere.
Storage Store dark at +2 to +8°C.
- Purpose: Media for vitrification of oocytes.
- Purpose: Media for warming of vitrified oocytes.
The oocyte - a fragile cell
In recent years, oocyte vitrification has become a reliabe routine for donation, as well as for fertility preservation, for medical and nonmedical reasons.
However, successful vitrification of an oocyte remains challenging. Compared to an embryo, which can develop with only half of the cells intact after cryopreservation, this possibility does not exist for an oocyte. Sufficient equilibration of the large cell volume with cryoprotectants is therefore absolutely crucial for survival and viability of the oocyte.
Special media for a unique cell
RapidVit Oocyte is a specialised calcium-free media for oocyte vitrification. The media was developed after extensive research on the effects of cryoprotectants on oocytes and their intracellular calcium content.
Studies show that careful control of intra- and extracellular calcium has a significant impact on the success of cryopreservation and oocyte viability.1,2
High lot-to-lot consistency ensures high performance
Vitrolife’s tests go well beyond regulatory demands, using multiple endpoint assessment including blastocyst cell number, with narrow acceptance levels to ensure high performance and the best results for the customers.
Vitrolife has unique competence in testing, which is performed in-house to maintain full control with traceability of each released product.
Save time in every procedure
Working at 37 °C shortens vitrification time and minimises exposure to cryoprotectants. Using RapidVit Oocyte will save you time in every procedure. The graphs compare the maximum times needed to vitrify oocytes using different brands.
Smooth workflow
Simply move the oocyte in a few short steps. Less handling reduces stress for the oocyte. A straightforward method is easy to learn and more standardised.
Excellent viability
Survival rates >90 % show that Rapid-i Vitrification System performs equally well as other systems.
“When overseeing multiple labs, repeatability becomes really important. We need a product that all embryologists can use easily.
The big win with RapidVit/Warm Oocyte and the Rapid-i is that it is a repeatable system with straightforward protocols and this enables us to get consistently good results with all our embryologists.”
- Lindsey Zujovic, Group Director of Embryology, TFP
A complete system
Rapid-i Vitrification System is an aseptic and closed system. It offers a simple, safe and structured workflow. Rapid-i Kit is renowned for being easy to load. Oocytes are held in place by surface tension - no sticking to the device.
Learn more about Rapid-i Vitrification System
References
- Larman MG, et al. Calcium-free vitrification reduces cryoprotectant-induced zona pellucida hardening and increases fertilization rates in mouse oocytes. Reproduction. 2006 Jan;131(1):53-61.
- Larman MG, et al. 1,2-propanediol and the type of cryopreservation procedure adversely affect mouse oocyte physiology. Hum Reprod. 2007 Jan;22(1):250-9. Epub 2006 Aug 12.
- Gardner D.K., Lane, M. Recombinant human serum albumin and hyaluronan can replace blood-derived albumin in embryo culture media. Fertility and Sterility 2000. 74 (Suppl) S31-S32.
- Larman et al (2007) Reproductive BioMedicine Online; www.rbmonline.com/Article/2987 Vol 15. No 6. 2007 692-700
- Pinasco M. et al. Oocyte Vitrification Freeze/Thaw Survival Rates Using an Open versus a Closed System, Fertility and Sterility, 2012, 97 (3), S18, P-27.
- Machac S. et al. A novel method for human oocyte vitrification with a closed device using super-cooled air, Fertility and Sterility, 2013, 100 (3), S108.
- Perez O. et al, Oocyte vitrification using a new vitrification medium and a new closed vitrification device. A sibling oocyte study, Fertility and Sterility, 2018, P-181 Vol. 110, Issue 4, e179-e180.
- Pujol A. et al. Comparison of two different oocyte vitrification methods: a prospective, paired study on the same genetic background and stimulation protocol, Human Reproduction, 2019, Vol.34, No.6, pp. 989-997.
- Vitrolife data-on-file, 2023 REP-6218 Clinical experience report: RapidVit Oocyte/RapidWarm Oocyte and Rapid-i Kit
- Barberet et al. 2022. Comparison of oocyte vitrification using a semi-automated or a manual closed system in human siblings: survival and transcriptomic analyses.
- Kurkkio and Bohlin 2022. Validation and clinical implementation of a fast, standardised and closed vitrification system for human oocytes.
- Vitrolife data-on-file, REP-4336-v.2.0 Design Validation Report - Rapid-i Kit.
Support Documents
Documents Language
Package inserts
English
Package insert RapidVit Oocyte
Document and Ref: REF 26280, version 06
Package inserts
English
Package insert RapidWarm Oocyte
Document and Ref: REF 26281, version 07
Safety data sheets (SDS)
English
SDS RapidVit Oocyte
Document and Ref: Date Revised : 06/27/2023. Revision No : 3. Safety data sheet for RapidVit Oocyte
Safety data sheets (SDS)
English
SDS RapidWarm Oocyte
Document and Ref: Date Revised : 06/27/2023. Revision No : 3. Safety data sheet for RapidWarm Oocyte
Short protocols
English
Rapid- i™ Vitrification of oocytes
Document and Ref: REF 19129. Version 03. Date of issue 220923. This short protocol describes how to vitrify and warm oocytes using Rapid-i™ vitrification system and RapidVit™/RapidWarm™ Oocyte.
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