G-2™ PLUS
G-2™ PLUS provides the embryo with a wide range of amino acids and hyaluronan for optimised blastocyst development.
Description G-2 PLUS is a bicarbonate buffered media containing hyaluronan. It also contains human serum albumin.
Purpose Medium for culture of embryos from day 3 to the blastocyst stage.
Storage Store dark at +2 to +8°C
- Description: Bicarbonate buffered medium containing hyaluronan and human serum albumin.
- Application: For use after equilibration at +37°C and 6 % CO2.
Appropriate nutrients to protect
and support
G-2 PLUS is a media for culture of human embryos from the 8-cell stage on day three until the blastocyst stage. G-2 PLUS is supplemented with human serum albumin, HSA, and is ready to use. It alos contains amino acids to support embryo viability and hyaluronan to improve development and cryosurvivability and to facilitate implantation. G-2 PLUS also contain vitamins that function as antioxidants to protect the embryo against reactive oxygen species during culture in vitro.
G-Series - confidence at each step
Each product in the G-Series™ is developed to resemble conditions in the female reproductive tract and fulfil embryo requirements.
Support Documents
Documents Language
Package inserts
English
Package insert G-2 PLUS - Multi-language
Document and Ref: REF 26082, version 09
Safety data sheets (SDS)
English
SDS G-2 PLUS
Document and Ref: Date Revised : 06/27/2023. Revision No : 6. Safety data sheet for G-2 PLUS
Short protocols
English
G-Series™ Embryo culture G-1 PLUS and G-2 PLUS
Document and Ref: REF 19183. Version 07. Date of issue 220923. This short protocol describes how to culture embryos from after fertilisation until the blastocyst stage using the G-Series™ sequential media G-1™ PLUS, G-2™ PLUS and OVOIL™.
Scientific summaries
English
Optimised clinical outcomes with blastocyst culture and cryopreservation using Vitrolife G-Series culture media
Document and Ref: A study by Zhu et al demonstrate that extended culture before cryopreservation allows for selection of viable embryos with a high chance of implantation after warming. The high blastocyst formation rate (73.8%) using Vitrolife culture media, together with an efficient cryopreservation program resulting in survival rates of >96% should eliminate any earlier concerns about blastocyst culture and cryopreservation.
Scientific summaries
English
Embryo culture in G-Series results in significantly higher cell numbers and implantation rates compared to culture in HTF
Document and Ref: A study by Kleijkers show that besides cell numbers and implantations rates, gene expression and certain pathways in preimplantation embryos are also affected by culture medium.
Short protocols
English
G-Series™ Blastocyst trophectoderm biopsy
Document and Ref: REF 19126. Version 02. Date of issue 220923. This short protocol describes how to perform blastocyst trophectoderm biopsy using OVOIL™ and G-Series™ media, G-2™ PLUS or G-TL™ and G-MOPS™ PLUS
Scientific summaries
English
G-Series culture media shown to increase blastocyst development compared to control
Document and Ref: In a study by Matsuoka et al, embryo culture was done either Vitrolife’s G-Series™ culture media or a mix of sequential culture media (control). The results show that good-quality blastocyst development was more frequent after culture in G-Series media containing hyaluronan.
Short protocols
English
Primo Vision™ time-lapse system - Embryo Culture using sequential media Method 1
Document and Ref: This short protocol describes how to use G-Series™ media G-MOPS™, G-MOPS PLUS, G-1™PLUS, G-2™ PLUS and OVOIL™ for handling of oocytes and embryo culture in Primo Vision™ time-lapse monitoring system and change medium on day 3 by preparing a new dish.
Short protocols
English
Primo Vision™ time-lapse system - Embryo Culture using sequential media Method 2
Document and Ref: This short protocol describes how to use G-Series™ media G-MOPS™, G-MOPS™ PLUS, G-1™PLUS, G-2™ PLUS and OVOIL™ for oocyte handling and embryo culture in Primo Vision time-lapse monitoring system and change medium on day 3 using the same dish.
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