HYASE-10X™
Facilitating denudation prior to ICSI procedures.
Description Physiological salt buffer containing hyaluronidase and human serum albumin.
Purpose Medium for removal of cumulus cells.
Application For use after dilution. See product insert.
Storage Store dark at +2 to +8°C
- Description: Containing gentamicin
Facilitating denudation
To be able to reach the oocyte and fulfill the mission of fertilisation, the spermatozoa release the enzyme hyaluronidase to make their way through the cumulus mass. In vitro, if ICSI is used as a fertilisation method, the cumulus cells must be removed by the embryologist. The same enzyme released by the spermatozoa is used.
When oocytes are placed in diluted HYASE-10X the cumulus mass is dispersed in a few seconds and the cells can easily be removed with a denudation pipette.
The concentration of hyaluronidase in HYASE-10X is 800 IU/mL
How we secure performance
Before releasing HYASE-10X, we simulate your work and your reality. Every batch of HYASE-10X is tested using Vitrolife’s proprietary MEA method. By placing mouse cumulus complexes in HYASE-10X for a set time, it is possible to check lot-to-lot consistency and enzyme activity by comparing with reference material.
Support Documents
Documents Language
Package inserts
English
Package insert HYASE-10x 10176 - Multi-language
Document and Ref: REF 26284, version 02
Safety data sheets (SDS)
English
SDS HYASE-10X
Document and Ref: Date Revised : 06/27/2023. Revision No : 6. Safety data sheet for HYASE-10X
Short protocols
English
G-Series™ ICSI procedure
Document and Ref: REF 19122. Version 02. Date of issue 220923. This short protocol describes how to perform ICSI using ICSI™, HYASE™-10x, OVOIL™ and G-Series medium G-MOPS™ PLUS .
Short protocols
English
Gx-Series™ ICSI procedure
Document and Ref: REF 19179. Version 1. Date of issue 220923 This short protocol describes how to perform ICSI using ICSI™, HYASE™-10x, OVOIL™ and Gx-Series medium Gx-MOPS™ PLUS and Gx-TL™
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