Facilitating denudation prior to ICSI procedures.
Description Physiological salt buffer containing hyaluronidase and human serum albumin.
Purpose Medium for removal of cumulus cells.
Application For use after dilution. See product insert.
Storage Store dark at +2 to +8°C
To be able to reach the oocyte and fulfill the mission of fertilisation, the spermatozoa release the enzyme hyaluronidase to make their way through the cumulus mass. In vitro, if ICSI is used as a fertilisation method, the cumulus cells must be removed by the embryologist. The same enzyme released by the spermatozoa is used.
When oocytes are placed in diluted HYASE-10X the cumulus mass is dispersed in a few seconds and the cells can easily be removed with a denudation pipette.
The concentration of hyaluronidase in HYASE-10X is 800 IU/mL
How we secure performance
Before releasing HYASE, we simulate your work and your reality. Every batch of HYASE is tested using Vitrolife’s proprietary MEA method. By placing mouse cumulus complexes in HYASE for a set time, it is possible check lot-to-lot consistency and enzyme activity by comparing with reference material.
Package insert HYASE-10x ref 10017
Package insert HYASE-10x ref 10176
Manual G-Series. International
This manual describes how to use all media in the G-Series: G-RINSE, G-MOPS, G-GAMETE, G-IVF, G-1, G-2, G-TL, G-PGD, HSA-solution, G-MM, ICSI, HYASEx-10x, OVOIL , SpermGrad and EmbryoGlue.
Manual G-Series. For US
This manual describes how to use all media in the G-Series: G-RINSE, G-MOPS, G-GAMETE, G-IVF, G-1, G-2, G-TL, G-PGD, HSA-solution, G-MM, ICSI, HYASEx-10x, OVOIL, SpermGrad and EmbryoGlue
Safety data sheets (SDS)
Safety data sheet for HYASE-10X
Short protocols - ICSI
This short protocol describes how to perform ICSI using ICSI™, HYASE™-10x, OVOIL™ and G-Series medium G-MOPS™ PLUS