SpermGrad™

Efficient separation for increased progressive motility.

Purpose For gradient sperm separation.

Storage Store dark at +2 to +8°C

Log in to purchase

SpermGrad™

1 x 125 mL
  • Description: Bicarbonate and HEPES buffered medium containing silane-coated, colloid silica particles 125 mL.
  • Application: For use after dilution with G-IVF™ PLUS or SpermRinse™.

SpermGrad™

1 x 30 mL
  • Description: Bicarbonate and HEPES buffered medium containing silane-coated, colloid silica particles 30 mL.
  • Application: For use after dilution with G-IVF™ PLUS or SpermRinse™.

SpermGrad™

2 x 30 mL
  • Description: Bicarbonate and HEPES buffered medium containing silane-coated, colloid silica particles. Upper (45%) and lower (90%) layer. 2 x 30 mL
  • Application: Ready to use solutions for gradient sperm separation.

SpermGrad™

2 x 125 mL
  • Description: Bicarbonate and HEPES buffered medium containing silane-coated, colloid silica particles. Upper (45%) and lower (90%) layer. 2 x 125 mL
  • Application: Ready to use solutions for gradient sperm separation.

Assortment that meets your need

The SpermGrad assortment contains both stock solutions and ready to use solutions, covering the needs from intermittent to high volume use - a time saver in an efficient laboratory. The stock solution is to be used after dilution with either G-IVF™ PLUS or with SpermRinse™.

Progressive motility correlates
to pregnancy rates

The goal for sperm separation is to select the sperm cells with the highest capacity of fertilising an oocyte. High quality sperm show progressive motility, which in clinical studies has been shown to correlate to pregnancy rates.1,2,3

Normal spermatozoa, abnormal spermatozoa, other cells present in the semen sample and bacteria all have different densities. SpermGrad contains silica coated particles that allow for separation of spermatozoa and other cells according to their density.

REF: 1. Jouannet P et al. Internation Journal of Andrology, 11:379-394, 1998. 2. Larsen L et al. Human Reproduction, 15:1562-1567, 2000. 3. Zinman MJ et al. Journal of Andrology, 21:145-153, 2000.

Efficient separation for increased progressive motility

High quality sperm show progressive motility which in clinical studies has been shown to correlate to pregnancy rates.1,2,3

SpermGrad™ is designed to support a successful treatment by an increased number of motile spermatozoa after preparation. This makes SpermGrad the gradient of choice during IVF treatment.

Clinical evaluation showed increased progressive motility

To investigate the quality of spermatozoa separated with SpermGrad, a clinical evaluation has been performed. The aim was to compare motility of the prepared samples and semen from 20 patients was included. The samples used for the comparison included severe male factor samples (<5 million/mL initial concentration with motility as low as 10%), as well as normal semen samples.

The data showed that sperm separated using SpermGrad gave a higher proportion of progressively motile sperm in the sample, compared to a leading brand1. This means that SpermGrad facilitates a better separation where a larger proportion of dead and degenerating spermatozoa have been removed.

REF: 1. Meintjes M. Frisco Institute for Reproductive Medicine. Data on file, 2012.

Select better sperm 

Sperm preparation with SpermGrad shows a positive effect on both average telomere length, sperm motility and DNA fragmentation removed.

Figure 1. The mean motile sperm rates, sperm DNA fragmentation rates, and average telomere length in raw semen and after processing by DGC using SpermGrad™. p<0.01 REF 1. Yang Q. et al. Processing of semen by density gradient selects spermatozoa with longer telomeres for assisted reproduction techniques. Reproductive BioMedicine Online (2015) 31, 44-50. 2. Yang Q., et al. Sperm telomere length is positively associated with the quality of early embryonic development. Human Reproduction (2015), 30 (8), 1876-1881

Support Documents

Documents Language

SpermGrad™

Package inserts

English

Package insert SpermGrad

Document and Ref: REF 20424, version 11

Gx-Series™

Short protocols

English

Gx-Series™ Sperm preparation - Density gradient centrifugation method

Document and Ref: REF 19177. Version 1. Date of issue 220923. This short protocol describes how to perform sperm preparation with the density gradient method and fertilization using products Gx-Series™ medium Gx-IVF™ PLUS or SpermGrad RTU and SpermGrad™

G-Series™

Short protocols

English

G-Series™ Sperm preparation - Density gradient centrifugation method

Document and Ref: REF 19120. Version 02. Date of issue 220923. This short protocol describes how to perform sperm preparation with the density gradient method and fertilization using products G-Series™ medium G-IVF™ PLUS and SpermGrad™

SpermGrad™

Scientific summaries

English

Sperm preparation with SpermGrad show positive effect on average telomere length, sperm motility and DNA fragmentation

Document and Ref: Yang Q, et al, found significant correlations between sperm telomere length and the quality of early embryonic development. Density gradient centrifugation using SpermGrad (Vitrolife) enrich for sperm with high mobility and low DNA fragmentation and is the preferred technique for selection of sperm with longer telomeres.

SpermGrad™

Safety data sheets (SDS)

English

SDS SpermGrad

Document and Ref: Date Revised : 06/27/2023. Revision No : 5. Safety data sheet for SpermGrad

Do you have a question about this product?

Contact us


Please add your professional email address.



Vitrolife needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at anytime. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our Privacy Policy.
Hidden




Find your local Vitrolife representative

Contact us

Maximise success every step of the way

We provide what you need to secure improved results throughout the IVF journey.