Short protocols
Document language
G-Series™ Blastocyst trophectoderm biopsy
Document ref and version: REF 19126. Version 02. Date of issue 220923. This short protocol describes how to perform blastocyst trophectoderm biopsy using OVOIL™ and G-Series™ media, G-2™ PLUS or G-TL™ and G-MOPS™ PLUS
G-Series™ Embryo culture G-1 PLUS and G-2 PLUS
Document ref and version: REF 19183. Version 07. Date of issue 220923. This short protocol describes how to culture embryos from after fertilisation until the blastocyst stage using the G-Series™ sequential media G-1™ PLUS, G-2™ PLUS and OVOIL™.
G-Series™ Embryo transfer - EmbryoGlue® 1x10 ml
Document ref and version: REF 19182. Version 03. Date of issue 221216 This short protocol describes how to perform embryo transfer using EmbryoGlue® 1x10 ml.
G-Series™ Embryo transfer - EmbryoGlue® 5x1.5 ml
Document ref and version: REF 19187. Version 3. Date of issue 220923. This short protocol describes how to perform embryo transfer using EmbryoGlue® 5x1.5 ml.
G-Series™ G-TL Embryo Culture
Document ref and version: REF 19125 version 1. Date of issure 2019-08-12. This short protocol describes how to perform embryo culture using G-TL™, OVOIL™ and G-MOPS™ PLUS.
G-Series™ ICSI procedure
Document ref and version: REF 19122. Version 02. Date of issue 220923. This short protocol describes how to perform ICSI using ICSI™, HYASE™-10x, OVOIL™ and G-Series medium G-MOPS™ PLUS .
G-Series™ Sperm preparation - Density gradient centrifugation method
Document ref and version: REF 19120. Version 02. Date of issue 220923. This short protocol describes how to perform sperm preparation with the density gradient method and fertilization using products G-Series™ medium G-IVF™ PLUS and SpermGrad™
G-Series™ Sperm preparation - Swim up method
Document ref and version: REF 19119. Version 02. Date of issue 220923. This short protocol describes how to perform sperm preparation with the swim-up method and fertilization of oocytes using G-series™ medium G-IVF™ PLUS
G-Series™ Oocyte retrieval and fertilisation
Document ref and version: REF 19121. Version 02. Date of issue 220923. This short protocol describes handling of oocytes and embryos in ambient atmosphere using G-Series™ media G-RINSE™, G-MOPS™, G-MOPS™ PLUS and G-IVF™ PLUS
Gx-Series™ Blastocyst trophectoderm biopsy
Document ref and version: REF 19180. Version 1. Date of issue 220923. his short protocol describes how to perform Blastocyst trophectoderm biopsy with OVOIL™, Gx-MOPS™ PLUS and Gx-TL™
Gx-Series™ Gx-TL Embryo Culture
Document ref and version: REF 19181. Version 1. Date of issue 220923. This short protocol describes how to perform embryo culture using Gx-TL™, OVOIL™ and Gx-MOPS™ PLUS.
Gx-Series™ ICSI procedure
Document ref and version: REF 19179. Version 1. Date of issue 220923 This short protocol describes how to perform ICSI using ICSI™, HYASE™-10x, OVOIL™ and Gx-Series medium Gx-MOPS™ PLUS and Gx-TL™
Gx-Series™ Sperm preparation - Density gradient centrifugation method
Document ref and version: REF 19177. Version 1. Date of issue 220923. This short protocol describes how to perform sperm preparation with the density gradient method and fertilization using products Gx-Series™ medium Gx-IVF™ PLUS or SpermGrad RTU and SpermGrad™
Gx-Series™ Sperm preparation - Swim up method
Document ref and version: REF 19176. Version 1. Date of issue 220923. This short protocol describes how to perform sperm preparation with the swim-up method and fertilization of oocytes using Gx-series™ medium Gx-IVF™
Gx-Series™ Oocyte retrieval and fertilisation
Document ref and version: REF 19178. Version 1. Date of issue 220923. This short protocol describes handling of oocytes and embryos in ambient atmosphere using Gx-Series™ media G-RINSE™, G-MOPS™, Gx-MOPS™ PLUS and Gx-IVF™ PLUS
Primo Vision™ time-lapse system - Dish loading
Document ref and version: This short protocol describes how to load Primo Vision™ dishes using 9-16 well dish.
Primo Vision™ time-lapse system - Embryo Culture using G-TL™
Document ref and version: REF 19124 Version 1 Date of issue 190312. This short protocol describes handling of embryos for culture in Primo Vision time-lapse system using G-Series™bmedia G-MOPS™, G-MOPS™ PLUS, OVOIL™ and G-TL™.
Primo Vision™ time-lapse system - Embryo Culture using sequential media Method 1
Document ref and version: This short protocol describes how to use G-Series™ media G-MOPS™, G-MOPS PLUS, G-1™PLUS, G-2™ PLUS and OVOIL™ for handling of oocytes and embryo culture in Primo Vision™ time-lapse monitoring system and change medium on day 3 by preparing a new dish.
Primo Vision™ time-lapse system - Embryo Culture using sequential media Method 2
Document ref and version: This short protocol describes how to use G-Series™ media G-MOPS™, G-MOPS™ PLUS, G-1™PLUS, G-2™ PLUS and OVOIL™ for oocyte handling and embryo culture in Primo Vision time-lapse monitoring system and change medium on day 3 using the same dish.
Primo Vision™ time-lapse system - Hardware set up
Document ref and version: This short protocol describes how to set up Primo Vision™ time-lapse monitoring system in a box incubator and connect to the computer.
Primo Vision™ time-lapse system - Start new project
Document ref and version: This short protocol describes how to start a new project (patient) in Primo Vision™ time-lapse monitoring system.
Rapid-i™ Vitrification System of blastocysts
Document ref and version: REF 19128. Version 03. Date of issue 220923. This short protocol describes how to vitrify and warm blastocysts using Rapid-i™ vitrification system and RapidVit™/RapidWarm™ Blast.
Rapid-i™ Vitrification of Cleavage stage embryos
Document ref and version: REF 19127. Version 03. Date of issue 220923. This short protocol describes how to vitrify and warm cleavage stage embryos using Rapid-i™ vitrification system and RapidVit™/RapidWarm ™ Cleave.
Rapid- i™ Vitrification of oocytes
Document ref and version: REF 19129. Version 03. Date of issue 220923. This short protocol describes how to vitrify and warm oocytes using Rapid-i™ vitrification system and RapidVit™/RapidWarm™ Oocyte.
Rapid-i™ Vitrification Omni. For all stages, from oocytes to blastocysts
Document ref and version: REF 19105. Version 02. Date of issue 220923. This short protocol describes how to vitrify and warm all cell stages from oocytes to blastocysts using Rapid-i ™vitrification system and RapidVit™/Warm™ Omni.
Slow freeze cryopreservation - Blasotcyst stage
Document ref and version: This short protocol describes how to Cryopreservation in Blasotcyst stage using HSA-solution™ or G-MM™, G-ThawKit Blast™, G-2™ PLUS.
Slow freeze cryopreservation - Cleavage stage embryos
Document ref and version: This short protocol describes how to slow-freeze zygotes and cleavage stage embryos using FreezeKit™ & ThawKit™ Cleave.
Sperm freezing when using cryovials
Document ref and version: REF 19023. Version 01. Date of issue 221201. This short protocol describes freezing of semen when using vials followed by gradient separation using and G-Series™ medium G-IVF™ PLUS and SpermGrad™.
Sperm freezing when using straws
Document ref and version: REF 19022. Version 01. Date of issue 221201. This short protocol describes freezing of semen when using straws followed by gradient separation using G-Series™ medium G-IVF™ PLUS and SpermGrad™.
